Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Examples: “Press carefully to engage,” “ Do not. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. > spectramax m4 microplate reader. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. DMSO was used to solubilize the formazan crystals and absorbance was measured at 570 nm (Spectramax M4, Molecular devices, USA). • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. DNA standards. 314 low lamp level warning. The Insulin standard curve was prepared as described in Section 10. Viability was determined by recording absorbance at 570 nm with plate reader (SpectraMax M4 Multimode, USA) and cell viability was determined. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Host your own live radio show with. * Nunc. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. In vitro transcription (IVT) is an essential technique for RNA synthesis. Bioz Stars score: 98/100, based on 1 PubMed citations. AM (~0. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. 3. 0 compensates for most changes in environmental temperature or pressure. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 4 Cell viability assay The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine cell viability; 1. The Multi-mode readers are top and bottom read. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. TEM. Published Dec 16, 2020 | Updated Dec 16, 2020. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. com. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Multi-Mode Microplate Readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 9000-0756. MTT values at day 3 were normalized to day 0. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Example: [Drawer]. Colony Formation Assay. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Each well was scanned at nine evenly distributed points and averaged automatically with the software. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. Optimized for fluorescence microplate readers. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Background-subtracted data values (mean +/- SD) are graphed. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Note: In this user guide, all references to SpectraMax Multi-Mode. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Results were normalized and expressed as a percentage of live cells. Turn the instrument around so that the back of the instrument is. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Background-subtracted data values (mean +/- SD) are graphed. After incubation, the absorbance value of all samples was measured at 517 nm on a Spectramax M4. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. SpectraMax® M4). Titration of human serum and plasma (heparin) within the working range of the assay. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Dagda Lab. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Highly selective for dsDNA over ssDNA or RNA. spr SpectraMax iD3 SpectraTest ABS2 v1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Bioz Stars score: 98/100, based on 1 PubMed citations. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. org) with optimized instrument settings (Table 1) were used to generate and analyze data. . The aim of the present study was to investigate the preventive effects of. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Roelli1, Amandine Stoss1, Rebecca A. The available phosphorus in the soil was leached by double-acid extraction (0. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. Plate chamber design of the SpectraMax category 1 microplate readers. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. The following document is intended to demonstrate setup of this instrument. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. Phillips5, Martin A. D 1 1. Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). SpectraMax® ABS. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. SigmaPlot (Systat Software, Inc. 0397 in x 3. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2. Thirty min later, each well was homogenized and the optical density (OD) was measured in a spectrometer (Spectramax M4, Molecular Devices) at 570 nm. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. To report an issue with this product, Brief content visible, double tap to read full content. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Next to the pushbutton is a green LED. 4 kilograms). The SpectraMax M2 and M2e are versatile and reliable. reader (SpectraMax M4, Molecular Devices; Sunnyvale, CA, USA) at 540 nm absorbance. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. Measurement of Mitochondrial Membrane Potential by JC-1. 7 cells. All experiments. 2. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Molecular Devices. Ltd. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. Dettmer4, Wayne A. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). Table of Contents. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). NCI-60 Human Tumor Cell Line Screen. Plot the fluorescence intensity versus the cell seeding number. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. WARNING! The instrument weighs approximately 36 pounds (16. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. TEM. The MTT reagent was incubated with cells for 4 h. Microplate Reader Shelf. 3 or greater. . Results were expressed as a percentage relative to the control group (defined as 100%). Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4To quantify F-CHP fluorescence, stained samples were digested in 500 μl of proteinase K (1 mg/ml) in water for 3 hours at 60°C. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). Images were taken using a fluorescence microscope (Nikon 80i, Japan). This platform allows the. . SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers; Instrument Field Depot; Aquamax 2000 Aquamax 2000/Stakmax. 1. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Background-subtracted data values (mean +/- SD) are graphed. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. Instruments Ltd. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). The plate reader optics are tunable for absorbance. 4. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). 5. StakMax Handling Stacker Base Unit. 2. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. Table of Contents. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. . Living cells were then incubated with 100 µl of medium assay provided by the assay kit. , San Jose, CA) was. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Lysosomal proteolytic activity. Microplate Reader Shelf. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Phillips5, Martin A. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. My List. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. . ). TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Detection modalities are shown in Table 1-1. SpectraMax® M4). 2. 1. 3. SpectraMax M4 Multi-Mode Microplate Reader. 1. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. SpectraMax® L (software version 5. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Standard features include a cuvette port,. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Serving Victoria's renovation and construction needs. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. Dumont3, Matthias S. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. /525 em. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Page: 1/6. SpectraMax® M5. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. After treatment, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, USA) was added to the PA and skatole treated hepatocytes and additionally incubated for 1 h. siRNA-mediated knockdown of KLF2 in RAW 264. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. High tolerance for contaminants. spr SpectraMax iD5 SpectraTest ABS2 v1. The whole process was protected from direct light. (Shanghai, China). Three kits covering a wide range of dsDNA concentrations. 116 SpectraMax M5 and M5e Multi-Mode. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. . However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. StakMax Handling Stacker Base Unit. 2. 2. 383 inches whereas the maximum allowed is 5. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). * BD Falcon® Microtest™ plates #353075 and # 353072). The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). Serial number location on Series II and Multi-Mode plate readers. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. 90-0083 series): Excitation 530 nm,. Bottom-reading is usually recommended for cell-based assay with adherent cells. 6: bioNova científica, s. STAKMAX. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. 1step assay with SpectraMax microplate readers. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. D 47 7. 7. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. The percentage of viability of each treatment group in different. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. Flasks containing 100 mL of YM broth were inoculated with 100. Lower limits of detection for ATP determined using the ATPlite . , UK) or SpectraMax M4 by Molecular Device. trophotometer (SpectraMax M4, Molecular Devices, USA). Description 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 7. Microplate Readers include the M3, M4, exception is noted. 2. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. Call 516-939-0888 to check current. 光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 140 多种预存实验方法可以直接调用，可以进行数 据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功 能，可以方便的进行各种数据结果的展示。 完整的. Control AM were incubated in the same buffer for 1 h at 37 °C. 5. Molecular Devices SpectraMax M4 User Manual (86 pages) Multi-Mode Microplate Readers. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Italic and boldface type are used for emphasis. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. Recertification is recommended every two years. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The SpectraMax M5e reader offers the additional benefit of being certified . Dissolve FITC-dextran in PBS at a. After preparation, wrap in foil (see Note 1). Analysis of flow cytometric samples was performed with BD FACSDiva software (BD Biosciences, San Jose, CA). Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. SpectraMax M3, M4, M5, and M5 e. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . . The MDD was determined by calculating the mean of zero standard replicates (n=24) and. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. . We have two NanoDrop micro-scale spectrophotometers, a Molecular Devices Spectramax M4 micro plate reader and a UVP gel documentation camera. All experiments. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. SpectraMax® iD5. +1 250-415-0785. Cellular Reactive Oxygen Species (ROS) was measured in Neuro2a cells seeded in 6-well plate at a density of 5000 cells/well. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). for Cisbio Bioassays. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. TSQ was dissolved in Lock’s buffer (pH 7. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Cells were exposed to drug solutions with. B07F4876MS. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. 2. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). 95. , and red fluorescence at 543 ex. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. 4. Highly selective for dsDNA over ssDNA or RNA. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. With optimized reagents, option to operate the. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. 5 mg mL −1 with 0. Moving a SpectraMax Multi-Mode Microplate Reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. item 7 replacement bulb for molecular devices spectramax m4 flash lamp replacement bulb for molecular devices spectramax m4 flash lamp. Description 1. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. 2. Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. Note: In this user guide, SpectraMax ® refers to several SpectraMax . While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Establishment of mouse orthotopic xenograft bladder tumor model. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Table 1. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. *Statistical significance: p < 0. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. Plot the fluorescence intensity versus the cell seeding number. 2. 1, BIO-RAD Laboratories). . Data analysis methods. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Brand: Molecular. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 7. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 306 plate air cal fail,low light. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. SpectraMax® ABS Plus. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Table 2. C. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. 1. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. free. 1. VIEW DETAILS. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Five hours prior to when permeability will be measured, gavage mice with 0. 2. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Description 1. SpectraMax Microplate Readers. 03 in x 3. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Description 1. Following digestion, 200 μl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485- and 525-nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). SpectraMax® Paradigm® Multi-Mode Detection Platform.